Biological screening of various medicinal plant extracts. In vitro antimicrobial activity of some medicinal plants. Antibacterial and antifungal activities from leaf extracts of. The antimicrobial activity of the test organisms to the 11 plant extracts was screened by using the agarwell diffusion method perez et al. These extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of ocimum basilicum l. The most active plant was torilis anthriscus especially in relation to pseudomonas glycinea. Screening of the ethnobotenical plants is a prerequisite to evaluate their therapeutic potential and it can lead to the isolation of new bioactive compounds. Plant derived medicines have made significant contributions towards human health and plants and are a great source of novel drug compounds. The present study aimed at evaluating the in vitro antimicrobial activity of methanolic extracts of some medicinal plants against escherichia coli, salmonella typhimurium, staphylococcus aureus and enterococcus sp.
The use of plant compounds to treat infection is an age. Antibacterial activities of plant extracts were compared with those of standard antibiotics. Antibacterial activity of aqueous extract of calotropis. Antimicrobial activity of some plant extracts against. Research article open access antibacterial activity of. Boiled water extract of neem leaf showed moderate zone of inhibition against escherichia coli 10 mm and. Antimicrobial activity of aqueous and organic extracts. The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments. All in all, data about specific antibiotic properties of coumarins are scarce, although many reports give reason to believe that some utility may reside in these phytochemicals 26, 83, 193.
Ates and others published antimicrobial activities of various medicinal and commercial plant extract find, read and cite all the. Species aegopodium podagraria, daucus carota, heracleum sphondylium and pimpinella. Antibacterial activities of some plant extracts utilized in popular medicine in palestine 102 1. Aquilaria crassna pierre ex lecomte has been traditionally used in thailand for treatment of infectious diseases such as diarrhoea and skin diseases for a long time. The methanolic extract phyllanthus niruri stone breaker showed the maximum activity against staphylococcus sp. Figure 3 minimal inhibitory concentration mic of plant extractsphytochemicals against antibiotic resistant bacteria. Antimicrobial activity, toxicity and phytochemical screening. Evaluation of antibacterial activity of some medicinal plants extracts. Antimicrobial activity of crude extracts of three medicinal plants used. In this study, nine plants extracts were screened for potential antibacterial activity table. Pdf on dec 5, 2011, elita scio and others published antimicrobial and antioxidant activities of some plant extracts find, read and cite all the research you. In vitro antifungal activity of dichloromethane and methanol extracts mgml. A study of antimicrobial activity of few medicinal herbs.
Phytochemicals, antioxidant and antimicrobial potentials. Antimicrobial activity of various extracts of ocimum. Increasingly, natural products have potential for the treatment of cancer. Antimicrobial susceptibility testing can be used for drug discovery, epidemiology and prediction of therapeutic outcome. Pdf antibacterial activity of medicinal plant extracts. Plant phenolic extracts as an effective strategy to control. Methods for in vitro evaluating antimicrobial activity. Phytochemical analysis of phyllanthus amarus extracts s. Evaluation of antibacterial activity of some medicinal plants. Antimicrobial potential of some plant extracts against. Aloe vera, antimicrobial activity, sap, leaves, minimum inhibitory concentration introduction. Antimicrobial activity of some plant extracts having hepatoprotective.
Screening of antibacterial activity of the medicinal plant. Microorganisms dichloromethane extracts methanol extracts ac mp sc ta ro pg ac mp sc ta ro pg. Antimicrobial activity of medicinal plant extracts and their synergistic effect on some selected pathogens desalegn amenu college of natural and computational science, biology department, wollega university, p. The worldwide increase of multidrug resistance in both community and healthcare associated bacterial infections has impaired the current antimicrobial therapy, warranting the search for other alternatives. Mics of aqueous extracts of both plants was between 12. Among the four extracts of neem, petroleum ether and chloroform extract showed strong antimicrobial activity against s. Pdf antimicrobial and antioxidant activities of some plant extracts.
Antimicrobial activity, toxicity and phytochemical. Research article open access antibacterial activity of some. Jun 03, 2015 evaluation of antibacterial activity of some medicinal plants used in sudanese folk medicine for treatment of gastrointestinal tract infections. Dried leaves of plants were mechanically ground, and 2 g of plant was extracted with 20 ml of acetone or chloroform or methanol, then it was gently heated. Antibacterial activity of some plant extracts nihal dog ru oz 1, zuhal zeybek 1, ali karagoz 2 1 istanbul university, faculty of science, department of b iology, 344, vezneciler, istanbultu. The methanol and aqueous extracts from different plant parts peel of fruits, rhizome, fruits, leaves, and gum respectively at a concentration of 100 mgml, were evaluated against 20 clinical. The extract of three medicinal plants used in nigerian folk medicine showed a highly antibacterial activity against some food borne pathogens. While some plant extracts were comparatively more effective against all the three pathogens xanthomonas campestris, agrobacterium rhizogenes and aspergillus fumigatus. Bioactive aqueous extracts of twelve yucatecan plants.
The differences of solvent related activity could be speculatively explained by the insolubility of active compounds in water. Chemical composition and antibacterial activity of essential. Studies on the antimicrobial activity of essential oils from t. Chemical composition and antibacterial activity of. The antimicrobial activities of some plant species phyllanthus emblica, tinospora cordifolia, eclipta alba, and cassia occidentalis extracts. The present study aimed at evaluating the in vitro antimicrobial activity of plant allium sativum, zingiber officinale, caryophyllus aromaticus, cymbopogon citratus, mikania glomerata and psidium guajava extracts against grampositive and gramnegative bacterial strains isolated from human infections. It was found that most plant extracts studied had antibacterial and antifungal activities. Antimicrobial activities of the crude ethanolic extracts of five plants were screened against multidrug resistant mdr strains of. Antimicrobial activity of medicinal plants against human. Different parts of the plants were collected, air dried, and powdered in a homogenizer, and 10 g of each plant was used for aqueous and ethanol extraction. The plant extracts were obtained by using ethanol and the concentration of the extract was 500600 mg l1 and the the inhibition zone was measured after 24 h at 30c. Evaluation of antibacterial activity of some traditionally. Antimicrobial activity of single and combined extracts of. Evaluation of antibacterial activity of these plant extracts was recorded in table 2 and illustrated in fig.
Studies on in vitro antimicrobial activity of ethanol extracts of rauvolfia tetraphylla. To determine antibacterial activity of water, oil and methanol extracts of guava psidium guajava, green tea camellia sinensis, neem azadirachta indica and marigold calendula officinalis against different species of bacteria, pseudomonas spp. Antimicrobial activity of wild olive crude extracts in vitro. Five plant species were investigated to evaluate their antibacterial activity against food poisoning bacteria including two strains of gram positive bacteria b. Pdf antimicrobial activities of various medicinal and commercial. Biochemistry graduates to have results from their final. Antimicrobial activity of some medicinal plant extracts against bacteria causing diarrhoea by naomi tope komolafe submitted in accordance with the requirements for the degree of master of science in the subject life sciences at the university of south africa supervisor.
Upon heating, it was rinsed for 24 h at room temperature. These results show that the aqueous extracts of five native plants of the. Extracts from a broad spectrum of plant species contain substances that possess antitumor activity 1. The results indicated that the methanol extracts of o. The present investigation was focused on screening of ethanol leaf extracts of catharanthus roseus from saudi arabia for its antibacterial and antifungal activity against number of pathogenic microorganisms. None of the aqueous extracts showed antimicrobial activity. Biochemistry graduates to have results from their final year. Plant extracts were added based on plackettburman design by varying the composition of chosen independent variables. Antimicrobial activity of selected medicinal plants.
Antimicrobial activity of some important medicinal plant. The study of antioxidant and antimicrobial activity of. An inoculum suspension was swabbed uniformly to solidified 20 ml muellerhinton agar mha for bacteria and sabouraud dextrose agar sda for fungi and the. Plant extracts with biofilm inhibitory activity biofilms are the default modeoflife for many bacterial species and biofilmbased infections cause harm to millions of humans annually. Plant extracts since many of the essential oils inhibited the growth of e. Antibacterial activity of medicinal plant extracts article pdf available in brazilian journal of microbiology 384. The methanolic extract of leaf of the plant azadiracta indica, acacia nilotica and witania somnifera showed significant. Environmental issues amoxicillin contamination ampicillin biological products fishes food contamination herbal medicine hostparasite relationships prevention materia medica, vegetable health aspects medicine, botanic. New drugs from plant secondary metabolites are being developed and studied for their antibacterial and anticancer activities 5. Antimicrobial activity of crude extracts of three medicinal. Research article open access antibacterial activity of some selected medicinal plants of pakistan yamin bibi1, sobia nisa1, fayyaz m chaudhary1 and muhammad zia2 abstract background.
Similarly, the nhexane and chloroform extracts of m. Sage and yarrow extracts did not present any antimicrobial activity. Antibacterial activity of extracts prepared from tropical and subtropical plants on methicillinresistant staphylococcus aureus. Natural matrices present multiple biological effects, being its antimicrobial potential in. Also, the mic values of real extracts have been determined as 20 0. Antibacterial and antifungal activities of plant part extracts against four pathogenic bacteria two grampositive and negative and three pathogenic fungi were investigated by the agar disk diffusion method. Antimicrobial activity of medicinal plant extracts n g r am et iv b c ada zwetlana, nandini m, kusuma dorcas abstract various types of antibacterial substances are present in higher plants. In relation to tetracycline activity, ethanol and ethyl acetate extracts showed similar or same activities while the water activity was higher than standard 100.
Antimicrobial activity of crude extracts of some plant leaves. Water extracts of green tea leaf at boiling and room temperature showed 17. Antibacterial activity of some plant extracts on salmonella. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity.
Antimicrobial activity of silver n anoparticles synthesized using plant extracts judita pui o 1, irena maionien 2, dovil jonkuvien, joana alomskien 1 department of physics, kaunas university of technology student 50, lt568 kaunas. Antimicrobial activity of some important medicinal plant against plant and human pathogens 12b. It is in view of this, that the present research was set up to evaluate the antimicrobial activity of, using different. Antibacterial activity the agar well diffusion method was performed to exploit antibacterial potential of used extracts 8. Antimicrobial activity of ethanol leaf extracts of. The three bacteria used in the current study were all gramnegative. Antibacterial and antifungal activities of thespesia populnea. Antimicrobial activity of some plant species used for the medical. Wild olive crude extracts were screened for antibacterial activity against five different bacterial human pathogens. Antibacterial activities of some borneo plant extracts. All extracts were stored at 4c till further analysis.
Four different solvents were used for the extraction. The study of antioxidant and antimicrobial activity of extracts for meat marinades 9690 romanian biotechnological letters, vol. These plants extracts were analyzed for elemental composition. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. All the plant extracts were screened for their antimicrobial activity. In vitro antimicrobial activity of some medicinal plants used by tribes. Biological screening of various medicinal plant extracts for. We aimed to find the in vitro antibacterial activity of ethanolic extracts of 16 different traditionally used medicinal plants of nepal against clinical and 2 reference bacterial. General antimicrobial activity was documented in woodruff galium odoratum extracts. Satish 1department of studies in applied botany and biotechnology, university of mysore, manasagangotri, mysore570 006 2herbal drug technology laboratory, department of. However, nhexane and chloroform fractions did not show any activity against a.
Antioxidant, antihypertensive, antihyperglycemic, and antimicrobial. Evaluation of antimicrobial activity of different solvent. Antimicrobial activity of medicinal plant extracts and. To prepare plant extracts three different solvents were used. Boiled water extracts of guava leaf showed the largest zone of inhibition 22 mm against v.
Antibacterial activity of plant extracts of alstonia scholaris. Abts, dpph and frap assays are widely used to determine the antioxidant capacity in plant extracts because of their simplicity, stability and accuracy. About 250,000 living plant species contain a much greater diversity of bioactive compounds than any chemical library made by humans but only few plant species have been systematically investigated for the presence of bioactive compounds wakdikar, 2004. Studies to determine the antibacterial acitivities of. Antimicrobial activity, pathogen microorganisms, medicinal plants.
Association of antibiotics and plant extracts showed synergistic antibacterial. Punica granatum, citrullus colocynthes, curcuma long, albizia lebbeckandazardirachta indicaare used in traditional medical practice in sudan for the treatment of gastrointestinal tract infections. Aug 20, 20 in addition to antioxidant activity, plant phenolic compounds, such as flavonoids and tannins, have been shown to possess antimicrobial activities 1618. Antimicrobial and antioxidant activity of essential oil and different. Studies on some plant extracts for their antimicrobial.
The highest antibacterial activity of crude eos was observed in p. Antibacterial and antifungal activities from leaf extracts. Antimicrobial activity of crude extracts from plant parts and corresponding calli of f bixa orellana l. Antibacterial activity of aquilaria crassna leaf extract.
Antimicrobial activity of selected medicinal plants against. The main objectives of this study were to examine antibacterial activity of the aquilaria crassna leaf extract against staphylococcus epidermidis and its underlying mechanism. Antibacterial and antifungal activities of thespesia. Pdf antimicrobial activity of medicinal plant leaf. Flowers and leaves extracts showed a powerful antimicrobial effect against all strains and the. Antibacterial activities of some plant extracts utilized. The methanolic extract of caryophyllus aromaticus presented the highest antis.
Antibacterial activity of some plant extracts on salmonella with special reference to its resistance pattern. Antimicrobial activity of medicinal plants against human pathogenic bacteria 657 inflammatory properties of plants mahesh and satish, 2008. The results showed that 16 extracts have antibacterial activity with more than 10 mm against a. Box, 395, ethiopia abstract antibiotics provide the main basis for the therapy of microbial bacterial and fungal infections. Antimicrobial assay the antimicrobial activity of the plant extracts was evaluated using agar well diffusion method 21 with minor modifications. Antimicrobial activity of medicinal plant leaf extracts. Antimicrobial activity of some plant extracts against bacterial strains. Antimicrobial activity of five herbal extracts against multi drug. The activity of crude essential oils against the three bacteria isolates was within the extremely sensitive category diameters of the inhibition zone larger than 20 mm after 24 and 48 hours of incubation table 1.
424 632 1166 424 314 1099 1559 26 306 858 32 481 595 937 1366 1454 624 619 995 244 804 740 1036 570 1019 1200 1523 1397 1395 1493 898 186 525 1212 1464 1375 897 1160 82 540 1398 314 468 1392 76 573 217 674 688